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KMID : 0861020090240040215
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Korea Journal of Herbology
2009 Volume.24 No. 4 p.215 ~ p.224
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Suppressive Effects of Chenilyeomgamibang (CGB) and Chenggihaedok-san (CHS) on TNCB(trinitrochlorobenzene)-induced Atopic Dermtitis NC/Nga Mice Model
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Lee Kyoung-Mee
Gim Seon-Bin
Choi Hak-Joo
Choi Jeong-June
Roh Seong-Soo
Kim Dong-Hee
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Abstract
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Objectives: Atopic dermatitis is a chronic inflammatory disease characterized by typically distributed eczematous skin lesion with pruritus, lichenification and dry skin. In this study, we performed to assess the therapeutic effects of co-treatment of Chenilyeomgamibang (CGB) and Chenggihaedok-san (CHS, C&C) on the TNCB(trinitrochlorobenzene)-induced atopic dermatitis in NC/Nga mice, characterized by the onset of atopic dermatitis along with an increase the number of inflammatory cells and dysregulation of Th2 cytokines.
Methods: Defined amount of CGB was sprayed on mice skin and CHS was simultaneously orally administrated to TNCB treated NC/Nga mice for 5 weeks. The immune cell types were caracterized by flow cytometry using each specific antibody. The amount of Th2 cytokines in serum and splenocytes culture supernatant were measured by ELISA.
Results: Administration of C&C significantly reduced clinical dermatitis severity including pruritus, edema, eczematous and erythema. Histological findings indicated that the thickening of epidermis/ dermis and dermal infiltration of inflammatory cells were dramatically reduced. Flow cytometry analysis showed that infiltrated immune cell numbers of CCR3+, B220+/IgE+, Gr-1+/CD11b+, and CD117+ were significantly reduced in C&C-treated dorsal skin lesion. Furthermore, T cell composition rate in PBMC was also dramatically decreased by the treatment. C&C greatly down-regulated production of Th2 cytokines including IL-4, IL-5, IL-13 in the serum. The down- regulatory effects of C&C on these Th2 cytokines production were also detected in CD3/ CD28 activated splenocytes.
Conclusions: These results indicated that C&C is a plausible therapeutic agent for treatment of atopic dermatitis through regulating the Th2 skewed immune system.
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KEYWORD
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Atopic dermatitis, Chenilyeomgamibang (CGB), Chenggihaedok-san (CHS), TNCB(trinitrochlorobenzene), NC/Nga mice, immune system
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